CISeAL researchers Carolina Portero, M.Sc., in collaboration with Dr. María Raquel Marchán-Rivadeneira, have developed a study that proposes an innovative strategy to combat SARS-CoV-2 infections through the development of synthetic probiotic bacteria capable of producing and releasing neutralizing nanoantibodies directly at the site of infection. The work is based on the premise that, although vaccines have been fundamental in controlling the pandemic, there is still a need for antiviral therapies that block early stages of the viral cycle, such as the binding of the RBD domain of the spike protein to the human ACE2 receptor, a key process for the virus to enter cells. In this context, nanoantibodies, derived from camelid antibodies, offer advantages over monoclonal antibodies due to their small size, stability, and ease of genetic manipulation, but they face limitations associated with their rapid degradation and the need for repeated administration. To overcome these challenges, a recombinant strain of Lactococcus lactis, a safe and widely used probiotic, was designed to act as a biological vehicle for producing and carrying nanoantibodies. Through genetic engineering, vectors capable of expressing the H11-D4 and H11-H4 nanoantibodies anchored to the bacterial surface were constructed, optimizing the secretion and fixation system to ensure their stability and external exposure.
The study conducted by Dr. Katherine Simbaña, Dr. Xavier Sánchez, and Dr. Ruth Jimbo Sotomayor, professors at the School of Medicine of PUCE and researchers at CISeAL, together with other experts, provides a global overview of the health effects of exposure to volcanic activity through a scoping review approach. The research is framed within a context where there are approximately 1,500 potentially active volcanoes worldwide, more than 550 of which have erupted in the past century, with a marked concentration in the Pacific Ring of Fire that runs through densely populated areas of Asia and the Americas. Volcanoes such as Fuego in Guatemala, Popocatépetl in Mexico, Tungurahua in Ecuador, and Merapi in Indonesia have produced highly explosive eruptions with major social and health consequences. The so-called “volcanic products,” which include ash, gases, pyroclastic flows, lava, and chronic emissions of pollutants such as carbon dioxide or radon, have differentiated health impacts depending on their physical and chemical properties, as well as on the duration and magnitude of exposure. In addition, external factors such as climate change, which intensifies secondary phenomena such as lahars or ash resuspension, and social determinants such as poverty, unplanned urbanization, and limited land-use planning significantly increase the vulnerability of populations living in high-risk areas.
The study, conducted using the scoping review methodology and following international guidelines such as PRISMA-ScR, included 83 articles after a rigorous selection process that began with 2,077 records identified in databases such as PubMed, Scopus, and Web of Science. The countries with the largest number of publications were the United States, Japan, and Iceland, and among the most studied volcanoes are Mount St. Helens, Kīlauea, Eyjafjallajökull, and Miyakejima. Most of the analyzed studies focused on adult populations, while research on children was less frequent. The most studied pollutants were toxic gases and volcanic ash, both responsible for a wide range of health effects.
This study, with the participation of CISeAL researcher Venus Medina, systematically analyzes support strategies for families of critically ill patients admitted to intensive care units (ICUs), focusing on the effectiveness of nursing interventions compared to standard care. The review covered articles published between 2015 and 2025 in databases such as PubMed, Scopus, CINAHL, SciELO, and Web of Science, following the PRISMA 2020 guidelines, and applied quality assessment tools such as the Johns Hopkins model and the ROB 2 instrument. Eighteen studies with diverse samples, although predominantly female, were included. These studies evaluated interventions aimed at communication, decision-making, and strengthening the emotional bond between family members and ICU patients. Strategies ranged from in-person programs—such as structured communication during rounds, open visitation policies, and family participation in care—to technology-mediated approaches that facilitated guidance and decision-making.
This study, with the participation of Dr. Venus Medina, principal investigator at CISeAL, addresses dating violence as an early manifestation of intimate partner violence, with significant consequences for health and social well-being. This phenomenon, characterized by its complexity, variability, and bidirectionality, requires methodologically rigorous instruments that allow for the precise identification of its dynamics among adolescents and young adults. The DVQ-VP, short for Dating Violence Questionnaire for Victimization and Perpetration, emerges as a culturally adapted and validated tool in Spanish-speaking populations, which assesses both victimization and perpetration across five dimensions: physical violence, psychological violence, sexual violence, controlling behaviors, and relational aggression.
Our principal investigator at CISeAL, Dr. Ruth Jimbo, participated in a study that reveals the high prevalence of neuropsychiatric disorders in Ecuadorian patients with central nervous system demyelinating diseases—multiple sclerosis (MS) and neuromyelitis optica spectrum disorder (NMOSD)—treated at the Carlos Andrade Marín Hospital in Quito, a referral center that concentrates around 60% of MS cases and 70% of NMOSD cases in the country. The study included 151 adult participants (117 with MS; 34 with NMOSD) who met current international diagnostic criteria. Sociodemographic data and disability status (EDSS) were documented, and psychiatric instruments validated in Ecuador (Hamilton, BDI‑II, MINI, CGI‑BP‑M, ISO‑30) were applied by trained psychologists. Women and mestizo populations predominated in both cohorts; NMOSD patients were on average older (49.8 vs. 42.3 years) and had greater disability (mean EDSS 4.23 vs. 2.5), with more cases in the moderate/severe range. The overall prevalence of neuropsychiatric disorders was high: 70.9% in MS and 85.29% in NMOSD. Moderate/severe anxiety was significantly more frequent in NMOSD, while depression showed comparable rates between groups.
The highest concentration of anxiety and depression was observed in the 41–50 age group in both diseases, but without statistically significant differences by age. Similarly, when stratified by disability, neuropsychiatric conditions clustered in mild disability in MS and moderate disability in NMOSD, without statistical significance. Problematic alcohol or substance use, as well as other diagnoses (bipolar disorder, social phobia, insomnia), was low; a low frequency of suicide attempts was also reported. These findings highlight the need for systematic screening and early multidisciplinary approaches to mental health in people with MS and NMOSD in the Latin American context.
We invite you to review the complete article for full details and results tables at the following link:We invite you to review the complete article for full details and results tables at the following link:
https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2025.1587804/full
The study analyzes the dynamics of antimalarial resistance in Plasmodium falciparum on the Pacific coast of Ecuador during 2019–2021, a critical period toward malaria pre-elimination, with emphasis on the influence of pharmacological pressure and human migration. The study was led by Dr. Fabián Sáenz, principal investigator of CISeAL, and was developed from the Bachelor's dissertation work of Isaac Ñacata in Biological Sciences. The work evaluated resistance genes (Pfcrt, Pfdhps, Pfdhfr, Pfmdr-1, Pfk13, and Pfaat1) in samples from the Esmeraldas and Carchi provinces and compared the frequencies of resistance mutations with data from 2013–2015. The results show a significant increase in haplotypes resistant to chloroquine (Pfcrt) and pyrimethamine (Pfdhfr), evidencing strong selective pressure. In contrast, sensitivity to sulfadoxine (Pfdhps) and artemisinin (Pfk13) was maintained, with the wild-type haplotype present in 100% of samples. In addition, the A16V mutation in Pfdhfr, linked to resistance to proguanil, was identified for the first time in Ecuador. The NEDFSDFY haplotype was also detected in Pfmdr-1, associated with multidrug resistance, reflecting the complexity of the resistance landscape.
Pancreatic adenocarcinoma represents a growing problem in Western countries due to its increasing incidence, difficult early diagnosis related to nonspecific symptoms, and intrinsic aggressiveness that translates into low overall survival (≈12% at 5 years), whereas neuroendocrine tumors (NETs) generally show a better prognosis and, in the pancreas, account for only ~2% of pancreatic neoplasms; the synchronous coexistence of both within the spectrum of multiple primary tumors (5–10% in the population) is exceptional. In this context, and with the participation of CISeAL principal investigators Dr. Ruth Jimbo-Sotomayor and Dr. Xavier Sanchez, we document the case of a 52-year-old man with type 2 diabetes who presented with three weeks of abdominal pain, weight loss, and anorexia without jaundice; laboratory studies showed mild hyperbilirubinemia, elevated transaminases, and a cholestatic pattern, and MRCP plus angiotomography revealed dilation of the bile duct and duct of Wirsung along with an 18 mm lesion in the pancreatic head without vascular invasion, clinically staged as IA. After ERCP biliary stent placement and preoperative evaluation, a robot-assisted cephalic duodenopancreatectomy (Whipple procedure) was performed, allowing precise dissection and lymphadenectomy; the postoperative course was uneventful. Histopathology identified a moderately differentiated adenocarcinoma with perineural and lymphovascular invasion, three negative nodes, and pathologic stage IB (pT2 pN0 pMX), as well as a separate incidental finding: a well-differentiated neuroendocrine tumor confined to the pancreatic head (pT1 pN0 pMX) with a low proliferative index, constituting a synchronous collision-type (biphasic) tumor and not a mixed neuroendocrine–nonendocrine neoplasm (MiNEN), in which both components should be admixed and each represent >30% of the mass. The patient received six cycles of FOLFOXIRI chemotherapy over eight months and then concurrent chemoradiotherapy with capecitabine (total dose 50.4 Gy at 13 months) and continues to do well, with no evidence of tumor activity on quarterly follow-up.
